Analysis Note

ControlHuman embryonic stem cells

Application

Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.

Immunofluorescence: A previous lot of this antibody was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.

Research CategoryStem Cell Research

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium Sulfate Precipitation

Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.

Format: Purified

Specificity

This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

Storage and Stability

Stable for 1 year at from date of receipt.

Target description

420/250 kDa